The aging of lithium-ion batteries (LIBs) is a crucial issue and must be investigated. The aging rate of LIBs depends not only on the material and electrochemical performance but also on the working conditions. In order to assess the impact of vehicle driving conditions, including the driving cycle, ambient temperature, charging mode, and trip distance on the battery life cycle, this paper first establishes an electric vehicle (EV) energy flow model to solve the operating parameters of the battery pack while working. Then, a powertrain test is carried out to verify the simulation model. Based on the simulated data under different conditions, the battery capacity fade process is estimated by using a semi-empirical aging model. The mileage (Ф) traveled by the vehicle before the end of life (EOL) of the battery pack is then calculated and taken as the evaluation index. The results indicate that the Ф is higher when the vehicle drives the Japanese chassis dynamometer test cycle JC08 than in the New European Driving Cycle (NEDC) and the Federal Test Procedure (FTP-75). The Ф will be dramatically reduced at both low and high ambient temperatures. Fast charging can increase the Ф at low ambient temperatures, whereas long trip driving can always increase Ф to varying degrees.